Generation of recombinant destabilase-lysozyme from medicinal leeches in three different expression systems.

@article{Manuvera2015GenerationOR,
  title={Generation of recombinant destabilase-lysozyme from medicinal leeches in three different expression systems.},
  author={Valentin A. Manuvera and Alexey S. Kurdyumov and Kseniya A Filonova and Vassili N. Lazarev},
  journal={Protein expression and purification},
  year={2015},
  volume={116},
  pages={
          50-8
        }
}
Destabilase-lysozyme (mlDL) is an enzyme secreted by the salivary gland cells of medicinal leeches. Destabilase-lysozyme possesses lysozyme and isopeptidase activities. We generated recombinant destabilase-lysozyme isoform 2 in three expression systems, i.e., in the bacteria Escherichia coli, in the yeast Pichia pastoris, and in the human cell line Expi293F. In E. coli, we generated both polypeptide in inclusion bodies that was later undergone to the refolding and soluble protein that had been… Expand
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