Generation of recombinant adeno-associated virus (rAAV) from an adenoviral vector and functional reconstitution of the NADPH-oxidase.

@article{Thrasher1995GenerationOR,
  title={Generation of recombinant adeno-associated virus (rAAV) from an adenoviral vector and functional reconstitution of the NADPH-oxidase.},
  author={Adrian J. Thrasher and Mahesh de Alwis and Colin M Casimir and Christine Kinnon and Kristen M. Page and Jane S Lebkowski and Anthony W Segal and Roland J. Levinsky},
  journal={Gene therapy},
  year={1995},
  volume={2 7},
  pages={481-5}
}
The human parvovirus, adeno-associated virus-2 (AAV-2), has many attributes that recommend its use as a gene transfer vehicle, including a broad tissue tropism, the ability to integrate stably into the host genome, and efficient transduction of cells which proliferate slowly. However, application to human gene therapy is currently limited by existing methods for generation of recombinant AAV (rAAV), resulting in relatively low transducing titres. In an attempt to overcome some of these problems… CONTINUE READING