Generation of porcine diploid blastocysts after injection of spermatozoa grown in nude mice.

@article{Nakai2009GenerationOP,
  title={Generation of porcine diploid blastocysts after injection of spermatozoa grown in nude mice.},
  author={Michiko Nakai and Hiroyuki Kaneko and T Somfai and Naoki Maedomari and Manabu Ozawa and Junko Noguchi and Naomi Kashiwazaki and Kazuhiro Kikuchi},
  journal={Theriogenology},
  year={2009},
  volume={72 1},
  pages={2-9}
}
It is anticipated that the utilization of spermatogonia through testicular xenografting will open new avenues for the conservation of male gametes. With the aim of establishing this new technique for genetic preservation of pigs, we used it in combination with intracytoplasmic sperm injection (ICSI). Testicular tissues derived from neonatal piglets, which contained seminiferous cords consisting of only gonocytes/spermatogonia, were transplanted under the back skin of castrated nude mice… CONTINUE READING
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