Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system.

@article{Reynolds1992GenerationON,
  title={Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system.},
  author={B A Reynolds and Samuel Weiss},
  journal={Science},
  year={1992},
  volume={255 5052},
  pages={
          1707-10
        }
}
Neurogenesis in the mammalian central nervous system is believed to end in the period just after birth; in the mouse striatum no new neurons are produced after the first few days after birth. In this study, cells isolated from the striatum of the adult mouse brain were induced to proliferate in vitro by epidermal growth factor. The proliferating cells initially expressed nestin, an intermediate filament found in neuroepithelial stem cells, and subsequently developed the morphology and antigenic… 

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Progeny of adult rat neural stem cells, when co-cultured with primary neurons and astrocytes from neonatal hippocampus, develop into electrically active neurons and integrate into neuronal networks with functional synaptic transmission.

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TLDR
The evidence that neurogenesis occurs in the adult mammalian central nervous system (CNS) and that the adult CNS contains NSCs is reviewed.

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TLDR
This work modified procedures to grow differentiated neurons from adult rat hypothalamus and brain stem to have the immunological and electrophysiological properties of immature mitotic neurons and should be useful in a variety of future studies of neuronal differentiation and function.

Regeneration and Proliferation of Embryonic and Adult Rat Hippocampal Neurons in Culture

TLDR
These are the first studies to demonstrate that over 50% of adult CNS cells with neuron-like characteristics retain regenerative and proliferative potential.

Identification of a multipotent astrocytic stem cell in the immature and adult mouse brain.

TLDR
It is concluded that environmental factors, simulated by certain in vitro conditions, transiently confer NSC-like attributes on astrocytes during a critical period in CNS development.
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