Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system.

  title={Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system.},
  author={B A Reynolds and Samuel Weiss},
  volume={255 5052},
Neurogenesis in the mammalian central nervous system is believed to end in the period just after birth; in the mouse striatum no new neurons are produced after the first few days after birth. In this study, cells isolated from the striatum of the adult mouse brain were induced to proliferate in vitro by epidermal growth factor. The proliferating cells initially expressed nestin, an intermediate filament found in neuroepithelial stem cells, and subsequently developed the morphology and antigenic… 

Neuronal stem cells in the brain of adult vertebrates

The neuronal precursors of the adult brain could be used as a source of cells for neuronal transplantation and could be manipulated in vivo or in vitro to introduce genes into the brain.

Neural stem cells from adult hippocampus develop essential properties of functional CNS neurons

Progeny of adult rat neural stem cells, when co-cultured with primary neurons and astrocytes from neonatal hippocampus, develop into electrically active neurons and integrate into neuronal networks with functional synaptic transmission.

Adult neurogenesis and neural stem cells of the central nervous system in mammals

The evidence that neurogenesis occurs in the adult mammalian central nervous system (CNS) and that the adult CNS contains NSCs is reviewed.

Characterization of mitotic neurons derived from adult rat hypothalamus and brain stem.

This work modified procedures to grow differentiated neurons from adult rat hypothalamus and brain stem to have the immunological and electrophysiological properties of immature mitotic neurons and should be useful in a variety of future studies of neuronal differentiation and function.

Regeneration and Proliferation of Embryonic and Adult Rat Hippocampal Neurons in Culture

These are the first studies to demonstrate that over 50% of adult CNS cells with neuron-like characteristics retain regenerative and proliferative potential.

Identification of a multipotent astrocytic stem cell in the immature and adult mouse brain.

It is concluded that environmental factors, simulated by certain in vitro conditions, transiently confer NSC-like attributes on astrocytes during a critical period in CNS development.



Proliferation and differentiation of neuronal stem cells regulated by nerve growth factor

It is reported that nerve growth factor controls the proliferation of neuronal precursors in a defined culture system of cells derived from the early embryonic brain.

Neuron Production in the Hippocampus and Olfactory Bulb of the Adult Rat Brain: Addition or Replacement? a

  • S. Bayer
  • Biology
    Annals of the New York Academy of Sciences
  • 1985
Recent comparing the patterns of adult neurogenesis in these two populations of the rat brain found the number of granule cells in the hippocampus and olfactory bulb to remain constant during adult life is summarized.

Synaptogenesis of cultured striatal neurons in serum-free medium: a morphological and biochemical study.

  • S. WeissJ. Pin J. Bockaert
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1986
It appears that the complete differentiation of striatal neurons in serum-free medium may provide a suitable model for the study of the physiological and regulatory mechanisms involved in nerve cell development.

Trophic stimulation of cultured neurons from neonatal rat brain by epidermal growth factor.

EGF was found to enhance survival and process outgrowth of primary cultures of subneocortical telencephalic neurons of neonatal rat brain in a dose-dependent manner and may act as a neurite elongation and maintenance factor for select neurons of the rodent central nervous system.

Postmitotic death is the fate of constitutively proliferating cells in the subependymal layer of the adult mouse brain

Low concentrations of a replication-deficient, recombinant retrovirus are injected directly into the lateral ventricles of adult mice for uptake by mitotically active subependymal cells, suggesting the fate of the postmitotic progeny is death.