Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA

Abstract

CRISPR/Cas mediated genome editing has been successfully demonstrated in mammalian cells and further applications for generating mutant mice were reported by injecting humanized Cas9 (hCas) mRNA and single guide RNA into fertilized eggs. Here we inject the circular plasmids expressing hCas9 and sgRNA into mouse zygotes and obtained mutant mice within a… (More)
DOI: 10.1038/srep03355

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