Generation of mouse chimeras with high contribution of tetraploid embryonic stem cells and embryonic stem cell-fibroblast hybrid cells.

@article{Matveeva2015GenerationOM,
  title={Generation of mouse chimeras with high contribution of tetraploid embryonic stem cells and embryonic stem cell-fibroblast hybrid cells.},
  author={Natalia M. Matveeva and Elena A Kizilova and Oleg L. Serov},
  journal={Methods in molecular biology},
  year={2015},
  volume={1313},
  pages={61-71}
}
The in vitro long-term cultivation of embryonic stem (ES) cells derived from pre-implantation embryos offers the unique possibility of combining ES cells with pre-implantation embryos to generate chimeras, thus facilitating the creation of a bridge between in vitro and in vivo investigations. Genomic manipulation using ES cells and homologous recombination… CONTINUE READING