Generation of monoclonal antibodies against autologous proteins in gene-inactivated mice.

@article{Declerck1995GenerationOM,
  title={Generation of monoclonal antibodies against autologous proteins in gene-inactivated mice.},
  author={Paul J Declerck and Peter Carmeliet and Margriet Verstreken and F de Cock and D{\'e}sir{\'e} Collen},
  journal={The Journal of biological chemistry},
  year={1995},
  volume={270 15},
  pages={
          8397-400
        }
}
Induction of an immune response is strongly dependent on the phylogenetic distance between antigen and recipient. In general, antibodies will not be raised against self-antigens nor against highly conserved domains. In the present study we describe the production and characterization of murine monoclonal "auto-antibodies" against murine tissue-type plasminogen activator (t-PA) raised in "knock-out" mice, homozygously deficient of the functional gene. 203 stable hybridomas were obtained… CONTINUE READING

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