Generation of micropatterned substrates using micro photopatterning.

@article{Doyle2009GenerationOM,
  title={Generation of micropatterned substrates using micro photopatterning.},
  author={Andrew D. Doyle},
  journal={Current protocols in cell biology},
  year={2009},
  volume={Chapter 10},
  pages={
          Unit 10.15
        }
}
Micro photopatterning (microPP) has been developed to rapidly test and generate different patterns for extracellular matrix adsorption without being hindered by the process of making physical stamps through nanolithography techniques. It uses two-photon excitation guided through a point-scanning confocal microscope to locally photoablate poly(vinyl) alcohol (PVA) thin films in user-defined computer-controlled patterns. PVA thin films are ideal for surface blocking, being hydrophilic substrates… CONTINUE READING
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