Generation of maize cell lines containing autonomously replicating maize streak virus-based gene vectors

@article{Palmer1999GenerationOM,
  title={Generation of maize cell lines containing autonomously replicating maize streak virus-based gene vectors},
  author={Kenneth E. Palmer and Jennifer Ann Thomson and Edward Peter Rybicki},
  journal={Archives of Virology},
  year={1999},
  volume={144},
  pages={1345-1360}
}
Virion sense gene replacement derivatives of maize streak virus (MSV) were constructed with selectable marker expression cassettes based on the bialaphos resistance gene (bar) and the CaMV 35S promoter. The effect on replication of increasing the genomic size was tested by including: (1) the 550-bp maize adh I intron and 68-bp TMV Ω RNA leader sequences upstream of the bar genes; and (2) a fusion between the bar and E. coli glutathione reductase (gor) genes. Three recombinant viral vectors… CONTINUE READING

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