Generation of knockout mice by Cpf1-mediated gene targeting

@article{Kim2016GenerationOK,
  title={Generation of knockout mice by Cpf1-mediated gene targeting},
  author={Yongsub Kim and Seung-A Cheong and Jong Geol Lee and Sei Kyung Chang and Myeong Sup Lee and In-Jeoung Baek and Young Hoon Sung},
  journal={Nature Biotechnology},
  year={2016},
  volume={34},
  pages={808-810}
}
1 associated with a higher birth rate (40.7%) than LbCpf1 (28.6%, Table 1). Although fertilized eggs from C57BL/6J mice are relatively sensitive to microinjection of engineered nuclease mRNAs5, these birth rates are suitable for gene targeting in C57BL/6J mice and similar to those observed To the Editor: Engineered nucleases, including transcription activator-like effector nucleases (TALENs) and CRISPR-Cas9, are under active development as versatile genome-editing tools in various organisms… CONTINUE READING
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