Generation of integration-free neural progenitor cells from cells in human urine

  title={Generation of integration-free neural progenitor cells from cells in human urine},
  author={Lihui Wang and Linli Wang and Wenhao Huang and Huanxing Su and Yanting Xue and Zhenghui Su and Baojian Liao and Haitao Wang and Xichen Bao and Dajiang Qin and Jufang He and Wutian Wu and Kwok-fai So and Guangjin Pan and Duanqing Pei},
  journal={Nature Methods},
Human neural stem cells hold great promise for research and therapy in neural disease. We describe the generation of integration-free and expandable human neural progenitor cells (NPCs). We combined an episomal system to deliver reprogramming factors with a chemically defined culture medium to reprogram epithelial-like cells from human urine into NPCs (hUiNPCs). These transgene-free hUiNPCs can self-renew and can differentiate into multiple functional neuronal subtypes and glial cells in vitro… 

Cellular Reprogramming of Human Peripheral Blood Cells

Conversion of adult human peripheral blood mononuclear cells into induced neural stem cell by using episomal vectors.

Reprogramming Fibroblasts to Neural Stem Cells by Overexpression of the Transcription Factor Ptf1a.

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Generating a Non-Integrating Human Induced Pluripotent Stem Cell Bank from Urine-Derived Cells

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Methods for the efficient conversion of blood cells into induced neurons (iNs) are described, including protocols to isolate cord blood CD133+ cells, infect them with Sendai virus vectors that express SOX2 and c-MYC, and differentiate the infected cells into mature neurons.

Human neural progenitors derived from integration-free iPSCs for SCI therapy

Neural stem and progenitor cells in health and disease

The regulation of NSPC fate and function, in the context of cell‐based disease models and patient‐specific cell‐replacement therapies, warrants review.

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The Sendai virus (SeV) provides an alternative integration-free reprogramming method that removes the danger of genetic alterations and enhances the prospects of iNSCs from bench to bedside and is potentially applicable in disease modeling in pigs and regenerative therapies in veterinary medicine.



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Direct reprogramming of Sertoli cells into multipotent neural stem cells by defined factors

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Direct reprogramming of mouse fibroblasts to neural progenitors

The simple yet powerful technique of induced pluripotency may eventually supply a wide range of differentiated cells for cell therapy and drug development. However, making the appropriate cells via

Human Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences

Results demonstrate that reprograming human somatic cells does not require genomic integration or the continued presence of exogenous reprogramming factors and removes one obstacle to the clinical application of human iPS cells.

Specification of neuronal and glial subtypes from human pluripotent stem cells

These studies reveal unique aspects of human cell biology, including intrinsically programmed developmental course, differential uses of transcription factors for neuroectoderm specification, and distinct responses to extracellular signals in regulating cell fate.