Generation of induced pluripotent stem cells from a small amount of human peripheral blood using a combination of activated T cells and Sendai virus

@article{Seki2012GenerationOI,
  title={Generation of induced pluripotent stem cells from a small amount of human peripheral blood using a combination of activated T cells and Sendai virus},
  author={Tomohisa Seki and Shinsuke Yuasa and Keiichi Fukuda},
  journal={Nature Protocols},
  year={2012},
  volume={7},
  pages={718-728}
}
Induced pluripotent stem cells (iPSCs) have become important cell sources for genetic disease models, and they have the potential to be cell sources for future clinical therapies. However, invasive tissue sampling reduces the number of candidates who consent to donate cells for iPSC generation. In addition, integrated transgenes can potentially insert at inappropriate points in the genome, and in turn have a direct oncogenic effect. Technical modifications using a combination of activated T… 
Generation of Induced Pluripotent Stem Cells from Human Peripheral T Cells Using Sendai Virus in Feeder-free Conditions
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TLDR
This induction method will be useful for the derivation of human integration‐free iPSCs and will also be applicable to the generation of iPSC‐derived neuronal cells for drug screening or therapeutics in the near future.
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Some of the main obstacles remaining to be overcome before successful application of human pluripotent stem cell-based therapy arrives in the clinic are discussed and what the future of stem cell research may look like are discussed.
Efficient generation of transgene- and feeder-free induced pluripotent stem cells from human dental mesenchymal stem cells and their chemically defined differentiation into cardiomyocytes.
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Safe iPSCs were reprogrammed from SCAP and DPSCs using non-integrating RNA virus vector, which is an RNA virus carrying no risk of altering host genome, and achieved the differentiation of hiPSCs to cardiomyocytes lineage under serum and feeder-free conditions, using a chemically defined medium CDM3.
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Current knowledge relating to both delivery systems and combinations of inducing factors including chemicals which are used to generate human iPS cells are covered.
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References

SHOWING 1-10 OF 23 REFERENCES
Derivation of Induced Pluripotent Stem Cells from Human Peripheral Blood T Lymphocytes
TLDR
The generation of T lymphocyte-derived iPSCs from small, clinically advantageous volumes of non-mobilized peripheral blood retain a normal karyotype and genetic identity to the donor.
Efficient generation of transgene-free human induced pluripotent stem cells (iPSCs) by temperature-sensitive Sendai virus vectors
TLDR
It is shown that Sendai virus vector, an RNA virus vector that carries no risk of integrating into the host genome, is a practical solution for the efficient generation of safer iPSCs and suggested that generation of transgene-free iPSCS from cord blood cells should be an important step in providing allogeneic iPSC-derived therapy in the future.
Reprogramming of human peripheral blood cells to induced pluripotent stem cells.
TLDR
A polycistronic vector encoding Oct4, Klf4, Sox2 and c-Myc is used to generate iPS cells from from frozen peripheral blood of several donors and these cells were derived from mature T cells as well as myeloid donor cells.
Efficient induction of transgene-free human pluripotent stem cells using a vector based on Sendai virus, an RNA virus that does not integrate into the host genome
TLDR
It is shown that Sendai virus (SeV), an RNA virus and carries no risk of altering host genome, is an efficient solution for generating safe iPSC and will accelerate the clinical application.
Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes
TLDR
It is shown that reprograming of juvenile human primary keratinocytes by retroviral transduction with OCT4, SOX2, KLF4 and c-MYC is at least 100-fold more efficient and twofold faster compared with reprogramming of human fibroblasts.
Generation of induced pluripotent stem cells from human blood.
TLDR
The ability to reprogram cells from human blood will allow the generation of patient-specific stem cells for diseases in which the disease-causing somatic mutations are restricted to cells of the hematopoietic lineage.
Suppression of induced pluripotent stem cell generation by the p53–p21 pathway
TLDR
Functional analyses of 34 p53-regulated genes demonstrate that the p53–p21 pathway serves as a barrier not only in tumorigenicity, but also in iPS cell generation in mouse and human fibroblasts.
Induction of Pluripotent Stem Cells from Mouse Embryonic and Adult Fibroblast Cultures by Defined Factors
TLDR
Induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions is demonstrated and iPS cells, designated iPS, exhibit the morphology and growth properties of ES cells and express ES cell marker genes.
Generation of induced pluripotent stem cells from human terminally differentiated circulating T cells.
A manuscript has appeared online demonstrating isolation of iPSCs from peripheral blood, including a single line that showed evidence for both TCR-β and TCR-γ rearrangement by PCR (Kunisato, A.,
Differentiation stage determines potential of hematopoietic cells for reprogramming into induced pluripotent stem cells
TLDR
It is shown that hematopoietic stem and progenitor cells give rise to iPS cells up to 300 times more efficiently than terminally differentiated B and T cells do, yielding reprogramming efficiencies of up to 28%.
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