Generation of induced pluripotent stem cells from bovine embryonic fibroblast cells

@article{Han2011GenerationOI,
  title={Generation of induced pluripotent stem cells from bovine embryonic fibroblast cells},
  author={Xiaoping Han and Jianyong Han and Fangrong Ding and Suying Cao and Seong Soo Lim and Yunping Dai and Ran Zhang and Yurui Zhang and Bing Lim and Ning Li},
  journal={Cell Research},
  year={2011},
  volume={21},
  pages={1509-1512}
}
Embryonic stem cell (ES cell) lines were first generated by culturing mouse inner cell mass (ICM) on feeder layers in 1981 [1]. However, in large domestic animals, attempts to establish ES cell lines from ICM of blastocysts or the later epiblast have not been successful. This has hindered the efficient production of genetically modified livestocks by using ES-based approaches. Recently, it was found that ectopic expression of various combinations of transcription factors is able to reprogram… CONTINUE READING