Generation of immunoliposomes using recombinant single-chain Fv fragments bound to Ni-NTA-liposomes.

@article{Rger2005GenerationOI,
  title={Generation of immunoliposomes using recombinant single-chain Fv fragments bound to Ni-NTA-liposomes.},
  author={Ronny R{\"u}ger and Dafne M{\"u}ller and Alfred Fahr and Roland E Kontermann},
  journal={Journal of drug targeting},
  year={2005},
  volume={13 7},
  pages={399-406}
}
Recombinant single-chain Fv antibody fragments (scFv) can be combined with liposomes to generate immunoliposomes for targeted drug delivery. Recent studies have shown that scFv molecules modified to express a C-terminal cysteine residue can be used for site-directed chemical conjugation. Here, we present a new method by immobilizing scFv fragments via their C-terminal hexahistidyl-tag on liposomes containing Ni-NTA-lipids (Ni-NTA-DOGS) in their lipid bilayer without the need to introduce… CONTINUE READING

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