Generation of iPSCs from mouse fibroblasts with a single gene, Oct4, and small molecules

@article{Li2011GenerationOI,
  title={Generation of iPSCs from mouse fibroblasts with a single gene, Oct4, and small molecules},
  author={Yanqin Li and Qiang Zhang and Xiaolei Yin and Weifeng Yang and Yuanyuan Du and Pingping Hou and Jian Ge and Chun Liu and Weiqi Zhang and Xu Zhang and Yetao Wu and Honggang Li and Kang Liu and Chen Wu and Zhihua Song and Yang Zhao and Yan Shi and H. Deng},
  journal={Cell Research},
  year={2011},
  volume={21},
  pages={196-204}
}
  • Yanqin Li, Qiang Zhang, +15 authors H. Deng
  • Published 2011
  • Biology, Medicine
  • Cell Research
The introduction of four transcription factors Oct4, Klf4, Sox2 and c-Myc by viral transduction can induce reprogramming of somatic cells into induced pluripotent stem cells (iPSCs), but the use of iPSCs is hindered by the use of viral delivery systems. Chemical-induced reprogramming offers a novel approach to generating iPSCs without any viral vector-based genetic modification. Previous reports showed that several small molecules could replace some of the reprogramming factors although at… Expand
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References

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TLDR
Valproic acid (VPA), a histone deacetylase inhibitor, enables reprogramming of primary human fibroblasts with only two factors, Oct4 and Sox2, without the need for the oncogenes c-Myc or Klf4, and supports the possibility of reprograming through purely chemical means. Expand
Reprogramming of murine fibroblasts to induced pluripotent stem cells with chemical complementation of Klf4
TLDR
A small-molecule screening platform applied to identify compounds that functionally replace the reprogramming factor Klf4 gave rise to iPS cells that are indistinguishable from murine embryonic stem cells, and can be used to screen large chemical libraries in search of novel compounds to replace theReprogramming factors that induce pluripotency. Expand
Induced Pluripotent Stem Cells Generated Without Viral Integration
TLDR
This work generated mouse induced pluripotent stem cells from fibroblasts and liver cells by using nonintegrating adenoviruses transiently expressing Oct4, Sox2, Klf4, and c-Myc, providing strong evidence that insertional mutagenesis is not required for in vitro reprogramming. Expand
Generation of Mouse Induced Pluripotent Stem Cells Without Viral Vectors
TLDR
The production of virus-free iPS cells, albeit from embryonic fibroblasts, addresses a critical safety concern for potential use of i PS cells in regenerative medicine. Expand
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TLDR
iPS cells competent for germline chimaeras can be obtained from fibroblasts, but retroviral introduction of c-Myc should be avoided for clinical application. Expand
Oct4-Induced Pluripotency in Adult Neural Stem Cells
TLDR
It is reported that exogenous expression of the germline-specific transcription factor Oct4 is sufficient to generate pluripotent stem cells from adult mouse NSCs and is demonstrated to be similar to embryonic stem cells in vitro and in vivo. Expand
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TLDR
Induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions is demonstrated and iPS cells, designated iPS, exhibit the morphology and growth properties of ES cells and express ES cell marker genes. Expand
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TLDR
This work sought to create a doxycycline-inducible lentiviral system to convert primary human fibroblasts and keratinocytes into human induced pluripotent stem cells (hiPSCs), and developed a strategy to induce hiPSC formation at high frequency. Expand
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TLDR
This protocol describes how to establish primary human fibroblasts lines and how to derive iPS cells by retroviral transduction of reprogramming factors, and takes 2 months to complete reprograming human primary fibro Blasts starting from biopsy. Expand
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TLDR
Three overall "reprogramming" strategies for the generation of pluripotent stem cells from somatic cells have been studied extensively, and the demonstration that iPS cells can be generated from mouse fibroblasts by the introduction of four transcription factors is underlined. Expand
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