Generation of iPSC line from patient with arrhythmogenic right ventricular cardiomyopathy carrying mutations in PKP2 gene.

@article{Khudiakov2017GenerationOI,
  title={Generation of iPSC line from patient with arrhythmogenic right ventricular cardiomyopathy carrying mutations in PKP2 gene.},
  author={Aleksandr Khudiakov and Daria Kostina and A. M. Zlotina and Natalia Yany and Alexey A. Sergushichev and Tatiana Pervunina and A. N. Tomilin and Anna Kostareva and Anna Malashicheva},
  journal={Stem cell research},
  year={2017},
  volume={24},
  pages={
          85-88
        }
}
Human iPSC line was generated from patient-specific adipose tissue-derived mesenchymal multipotent stromal cells carrying two mutations in plakophilin-2 (PKP2) gene using non-integrative reprogramming method. Reprogramming factors OCT4, KLF4, SOX2, CMYC were delivered using Sendai viruses. Pluripotency was confirmed in vitro using immunofluorescence and RT-PCR analysis and in vivo by teratoma assay. The reported iPSC line could be useful tool for in vitro modeling of arrhythmogenic right… CONTINUE READING