Generation of human monoclonal antibodies against HIV-1 proteins; electrofusion and Epstein-Barr virus transformation for peripheral blood lymphocyte immortalization.

@article{Buchacher1994GenerationOH,
  title={Generation of human monoclonal antibodies against HIV-1 proteins; electrofusion and Epstein-Barr virus transformation for peripheral blood lymphocyte immortalization.},
  author={A. Buchacher and R. Predl and K. Strutzenberger and W. Steinfellner and A. Trkola and M. Purtscher and G. Gruber and C. Tauer and F. Steindl and A. Jungbauer},
  journal={AIDS research and human retroviruses},
  year={1994},
  volume={10 4},
  pages={
          359-69
        }
}
  • A. Buchacher, R. Predl, +7 authors A. Jungbauer
  • Published 1994
  • Biology, Medicine
  • AIDS research and human retroviruses
  • Electrofusion and EBV transformation were studied by immortalizing human PBLs from blood of HIV-1-positive volunteers. A panel of 33 cell lines producing human monoclonal antibodies (Hu-MAbs) against HIV-1 was established by cell fusion or EBV transformation. For the first fusion experiments the source of B lymphocytes was peripheral blood of HIV-1-infected donors in CDC stages II or III with CD4 cell counts higher than 500/mm3. Later on, from these patients only, those with high anti-HIV… CONTINUE READING
    525 Citations
    Antigenic Properties of the Human Immunodeficiency Virus Transmembrane Glycoprotein during Cell-Cell Fusion
    • 71
    • PDF
    A broadly neutralizing human monoclonal antibody against gp41 of human immunodeficiency virus type 1.
    • 357

    References

    SHOWING 1-10 OF 32 REFERENCES
    Generation of human monoclonal antibodies to human immunodeficiency virus.
    • 163
    • PDF
    Human monoclonal antibody against glycoproteins of human immunodeficiency virus.
    • 17
    Production of site-selected neutralizing human monoclonal antibodies against the third variable domain of the human immunodeficiency virus type 1 envelope glycoprotein.
    • 153
    • PDF
    Human-human hybridomas producing monoclonal antibodies of predefined antigenic specificity.
    • L. Olsson, H. Kaplan
    • Biology, Medicine
    • Proceedings of the National Academy of Sciences of the United States of America
    • 1980
    • 355
    • PDF