Generation of human monoclonal antibodies against HIV-1 proteins; electrofusion and Epstein-Barr virus transformation for peripheral blood lymphocyte immortalization.

@article{Buchacher1994GenerationOH,
  title={Generation of human monoclonal antibodies against HIV-1 proteins; electrofusion and Epstein-Barr virus transformation for peripheral blood lymphocyte immortalization.},
  author={A. Buchacher and R. Predl and K. Strutzenberger and W. Steinfellner and A. Trkola and M. Purtscher and G. Gruber and C. Tauer and F. Steindl and A. Jungbauer},
  journal={AIDS research and human retroviruses},
  year={1994},
  volume={10 4},
  pages={
          359-69
        }
}
  • A. Buchacher, R. Predl, +7 authors A. Jungbauer
  • Published 1994
  • Biology, Medicine
  • AIDS research and human retroviruses
    • Electrofusion and EBV transformation were studied by immortalizing human PBLs from blood of HIV-1-positive volunteers. A panel of 33 cell lines producing human monoclonal antibodies (Hu-MAbs) against HIV-1 was established by cell fusion or EBV transformation. For the first fusion experiments the source of B lymphocytes was peripheral blood of HIV-1-infected donors in CDC stages II or III with CD4 cell counts higher than 500/mm3. Later on, from these patients only, those with high anti-HIV… CONTINUE READING
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