Generation of human induced pluripotent stem cells from urine samples
@article{Zhou2012GenerationOH, title={Generation of human induced pluripotent stem cells from urine samples}, author={Ting Zhou and Christina Benda and Sarah Dunzinger and Yinghua Huang and Jenny Cy Ho and Jiayin Yang and Yu Wang and Ya Zhang and Qiang Zhuang and Yanhua Li and Xichen Bao and Hung-Fat Tse and Johannes Grillari and Regina Grillari-Voglauer and Duanqing Pei and Miguel Angel Esteban}, journal={Nature Protocols}, year={2012}, volume={7}, pages={2080-2089} }
Human induced pluripotent stem cells (iPSCs) have been generated with varied efficiencies from multiple tissues. Yet, acquiring donor cells is, in most instances, an invasive procedure that requires laborious isolation. Here we present a detailed protocol for generating human iPSCs from exfoliated renal epithelial cells present in urine. This method is advantageous in many circumstances, as the isolation of urinary cells is simple (30 ml of urine are sufficient), cost-effective and universal…
464 Citations
A non-invasive method to generate induced pluripotent stem cells from primate urine
- BiologybioRxiv
- 2020
This study introduces a novel and efficient approach to generate iPSCs non-invasively from primate urine, which will allow to extend the zoo of species available for a comparative approach to molecular and cellular phenotypes.
Improvement of transfection with reprogramming factors in urinederived cells
- BiologyBIOCELL
- 2020
An experimental protocol for obtaining and generating iPS-like cells from urine samples for further cell therapy research on different human diseases is provided and it is concluded that Lipofectamine Stem Cell transfection reagent is more effective than FuGENE in obtaining i PSCs under the conditions tested.
Generating a Non-Integrating Human Induced Pluripotent Stem Cell Bank from Urine-Derived Cells
- BiologyPloS one
- 2013
Using the approach described in this study, 93 iPS cell lines from 20 donors with diverse genetic backgrounds are generated and it is shown that this approach could be applied in a large population with different genetic backgrounds.
Generation of infant- and pediatric-derived urinary induced pluripotent stem cells competent to form kidney organoids
- Medicine, BiologyPediatric Research
- 2019
Urine in small volumes or collected in bags is a reliable source for reprogrammable somatic cells that can be utilized to generate kidney organoids, and constitutes an attractive approach for patient-specific iPSC research involving infants and children with wide applicability and a low threshold for participation.
Generation of Human Induced Pluripotent Stem Cells from Renal Epithelial Cells.
- BiologyMethods in molecular biology
- 2021
A stepwise method developed in the laboratory for the generation of iPSCs from renal epithelial cells present in urine, which is noninvasive, nonintegrating, and universal is depicted.
Human Induced Pluripotent Stem (hiPS) Cells from Urine Samples: A Non‐Integrative and Feeder‐Free Reprogramming Strategy
- BiologyCurrent protocols in human genetics
- 2017
This unit describes a protocol combining both of these advantages to generate hiPS cells with episomal plasmid transfection from urine samples of individuals carrying the desired genotype, generated rapidly (<10 weeks) and with high efficiency.
Generation of Mesenchymal-Like Stem Cells From Urine in Pediatric Patients.
- Biology, MedicineTransplantation proceedings
- 2016
Urine-derived cells provide a readily accessible cell type for feeder-free mRNA reprogramming
- Biology, Computer ScienceScientific Reports
- 2018
It is shown that mRNA reprogramming efficiently generates hiPSCs from urine-derived cells, which will contribute to accelerating the translation of hiPSC protocols into clinical treatment and to therapeutic applications.
Generation of human induced pluripotent stem cells from urinary cells of a healthy donor using a non-integration system.
- Biology, MedicineStem cell research
- 2017
Human Urinary Epithelial Cells as a Source of Engraftable Hepatocyte-Like Cells Using Stem Cell Technology
- Biology, MedicineCell transplantation
- 2016
Reprogramming of human urinary epithelial cells into iPSCs and subsequent hepatic differentiation, followed by a detailed characterization of the newly generated iHeps resulted in a large number of hepatocyte-preferred genes, including nuclear receptors that regulate genes involved in cholesterol homeostasis, bile acid transport, and detoxification.
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