Generation of human induced pluripotent stem cells from urine samples

  title={Generation of human induced pluripotent stem cells from urine samples},
  author={Ting Zhou and Christina Benda and Sarah Dunzinger and Yinghua Huang and Jenny Cy Ho and Jiayin Yang and Yu Wang and Ya Zhang and Qiang Zhuang and Yanhua Li and Xichen Bao and Hung-Fat Tse and Johannes Grillari and Regina Grillari-Voglauer and Duanqing Pei and Miguel Angel Esteban},
  journal={Nature Protocols},
Human induced pluripotent stem cells (iPSCs) have been generated with varied efficiencies from multiple tissues. Yet, acquiring donor cells is, in most instances, an invasive procedure that requires laborious isolation. Here we present a detailed protocol for generating human iPSCs from exfoliated renal epithelial cells present in urine. This method is advantageous in many circumstances, as the isolation of urinary cells is simple (30 ml of urine are sufficient), cost-effective and universal… 

A non-invasive method to generate induced pluripotent stem cells from primate urine

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Improvement of transfection with reprogramming factors in urinederived cells

An experimental protocol for obtaining and generating iPS-like cells from urine samples for further cell therapy research on different human diseases is provided and it is concluded that Lipofectamine Stem Cell transfection reagent is more effective than FuGENE in obtaining i PSCs under the conditions tested.

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Generation of infant- and pediatric-derived urinary induced pluripotent stem cells competent to form kidney organoids

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Generation of Human Induced Pluripotent Stem Cells from Renal Epithelial Cells.

A stepwise method developed in the laboratory for the generation of iPSCs from renal epithelial cells present in urine, which is noninvasive, nonintegrating, and universal is depicted.

Human Induced Pluripotent Stem (hiPS) Cells from Urine Samples: A Non‐Integrative and Feeder‐Free Reprogramming Strategy

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Generation of Mesenchymal-Like Stem Cells From Urine in Pediatric Patients.

Urine-derived cells provide a readily accessible cell type for feeder-free mRNA reprogramming

It is shown that mRNA reprogramming efficiently generates hiPSCs from urine-derived cells, which will contribute to accelerating the translation of hiPSC protocols into clinical treatment and to therapeutic applications.

Human Urinary Epithelial Cells as a Source of Engraftable Hepatocyte-Like Cells Using Stem Cell Technology

Reprogramming of human urinary epithelial cells into iPSCs and subsequent hepatic differentiation, followed by a detailed characterization of the newly generated iHeps resulted in a large number of hepatocyte-preferred genes, including nuclear receptors that regulate genes involved in cholesterol homeostasis, bile acid transport, and detoxification.



Generation of induced pluripotent stem cells from human cord blood cells with only two factors: Oct4 and Sox2

A detailed protocol for the derivation of CB stem cells and how they can be reprogrammed to pluripotency by retroviral transduction with only two factors (OCT4 and SOX2) in 2 weeks and without the need for additional chemical compounds is presented.

Pluripotency can be rapidly and efficiently induced in human amniotic fluid-derived cells.

Direct reprogramming of human somatic cells into pluripotency has broad implications in generating patient-specific induced pluripotent stem (iPS) cells for disease modeling and cellular replacement

Generation of human-induced pluripotent stem cells

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Generation of Human Induced Pluripotent Stem Cells from Umbilical Cord Matrix and Amniotic Membrane Mesenchymal Cells*

The efficient generation of induced pluripotent stem cells (iPSCs) from mesenchymal cells of the umbilical cord matrix and the placental amniotic membrane is reported using exogenous factors and a chemical mixture.

Feeder-free derivation of induced pluripotent stem cells from adult human adipose stem cells

It is shown here that induced pluripotent stem cells can be generated from adult human adipose stem cells (hASCs) freshly isolated from patients, and can be readily derived from adult hASCs in a feeder-free condition, thereby eliminating potential variability caused by using feeder cells.

Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes

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Mouse Meningiocytes Express Sox2 and Yield High Efficiency of Chimeras after Nuclear Reprogramming with Exogenous Factors*

It is demonstrated that cells from the mouse meningeal membranes express elevated levels of the embryonic master regulator Sox2 and are highly amenable to iPS, highlighting a population of somatic cells that are ready to undergo i PS, thus highlighting a very attractive cell type for iPS research and application.

Generation of Induced Pluripotent Stem Cells from Human Renal Proximal Tubular Cells with Only Two Transcription Factors, Oct4 and Sox2*

This work describes the rapid, reproducible, and highly efficient generation of iPSCs derived from endogenous kidney tubular renal epithelial cells with only two transcriptional factors, OCT4 and SOX2.

Generation of endoderm‐derived human induced pluripotent stem cells from primary hepatocytes

The technology to develop endoderm–derived human iPS cell lines will provide a foundation for elucidating the mechanisms of cellular reprogramming and for studying the safety and efficacy of differentially originatedhuman iPS cells for cell therapy.