• DOI:10.1038/nprot.2012.115
Corpus ID: 205465442

Generation of human induced pluripotent stem cells from urine samples

@article{Zhou2012GenerationOH,
  title={Generation of human induced pluripotent stem cells from urine samples},
  author={Ting Zhou and C. Benda and S. Dunzinger and Yinghua Huang and J. C. Ho and Jiayin Yang and Yu Wang and Y. Zhang and Qiang Zhuang and Yanhua Li and X. Bao and H. Tse and J. Grillari and R. Grillari‐Voglauer and D. Pei and M. A. Esteban},
  journal={Nature Protocols},
  year={2012},
  volume={7},
  pages={2080-2089}
}

• Ting Zhou, C. Benda, +13 authors M. A. Esteban
• Published 2012
• Biology, Medicine
• Nature Protocols

Human induced pluripotent stem cells (iPSCs) have been generated with varied efficiencies from multiple tissues. Yet, acquiring donor cells is, in most instances, an invasive procedure that requires laborious isolation. Here we present a detailed protocol for generating human iPSCs from exfoliated renal epithelial cells present in urine. This method is advantageous in many circumstances, as the isolation of urinary cells is simple (30 ml of urine are sufficient), cost-effective and universal… Expand