Generation of human induced pluripotent stem cells from urine samples

@article{Zhou2012GenerationOH,
  title={Generation of human induced pluripotent stem cells from urine samples},
  author={Ting Zhou and Christina Benda and Sarah Dunzinger and Yinghua Huang and Jenny Cy Ho and Jiayin Yang and Yu Wang and Ya Zhang and Qiang Zhuang and Yanhua Li and Xichen Bao and Hung-Fat Tse and Johannes Grillari and Regina Grillari‐Voglauer and Duanqing Pei and Miguel Angel Esteban},
  journal={Nature Protocols},
  year={2012},
  volume={7},
  pages={2080-2089}
}
Human induced pluripotent stem cells (iPSCs) have been generated with varied efficiencies from multiple tissues. Yet, acquiring donor cells is, in most instances, an invasive procedure that requires laborious isolation. Here we present a detailed protocol for generating human iPSCs from exfoliated renal epithelial cells present in urine. This method is advantageous in many circumstances, as the isolation of urinary cells is simple (30 ml of urine are sufficient), cost-effective and universal… 

A non-invasive method to generate induced pluripotent stem cells from primate urine

This study introduces a novel and efficient approach to generate iPSCs non-invasively from primate urine, which will allow to extend the zoo of species available for a comparative approach to molecular and cellular phenotypes.

Improvement of transfection with reprogramming factors in urinederived cells

An experimental protocol for obtaining and generating iPS-like cells from urine samples for further cell therapy research on different human diseases is provided and it is concluded that Lipofectamine Stem Cell transfection reagent is more effective than FuGENE in obtaining i PSCs under the conditions tested.

Generating a Non-Integrating Human Induced Pluripotent Stem Cell Bank from Urine-Derived Cells

Using the approach described in this study, 93 iPS cell lines from 20 donors with diverse genetic backgrounds are generated and it is shown that this approach could be applied in a large population with different genetic backgrounds.

Generation of infant- and pediatric-derived urinary induced pluripotent stem cells competent to form kidney organoids

Urine in small volumes or collected in bags is a reliable source for reprogrammable somatic cells that can be utilized to generate kidney organoids, and constitutes an attractive approach for patient-specific iPSC research involving infants and children with wide applicability and a low threshold for participation.

Generation of Human Induced Pluripotent Stem Cells from Renal Epithelial Cells.

A stepwise method developed in the laboratory for the generation of iPSCs from renal epithelial cells present in urine, which is noninvasive, nonintegrating, and universal is depicted.

Human Induced Pluripotent Stem (hiPS) Cells from Urine Samples: A Non‐Integrative and Feeder‐Free Reprogramming Strategy

This unit describes a protocol combining both of these advantages to generate hiPS cells with episomal plasmid transfection from urine samples of individuals carrying the desired genotype, generated rapidly (<10 weeks) and with high efficiency.

Generation of Mesenchymal-Like Stem Cells From Urine in Pediatric Patients.

Urine-derived cells provide a readily accessible cell type for feeder-free mRNA reprogramming

It is shown that mRNA reprogramming efficiently generates hiPSCs from urine-derived cells, which will contribute to accelerating the translation of hiPSC protocols into clinical treatment and to therapeutic applications.

Human Urinary Epithelial Cells as a Source of Engraftable Hepatocyte-Like Cells Using Stem Cell Technology

Reprogramming of human urinary epithelial cells into iPSCs and subsequent hepatic differentiation, followed by a detailed characterization of the newly generated iHeps resulted in a large number of hepatocyte-preferred genes, including nuclear receptors that regulate genes involved in cholesterol homeostasis, bile acid transport, and detoxification.
...

References

SHOWING 1-10 OF 62 REFERENCES

Generation of induced pluripotent stem cells from human cord blood cells with only two factors: Oct4 and Sox2

A detailed protocol for the derivation of CB stem cells and how they can be reprogrammed to pluripotency by retroviral transduction with only two factors (OCT4 and SOX2) in 2 weeks and without the need for additional chemical compounds is presented.

Pluripotency can be rapidly and efficiently induced in human amniotic fluid-derived cells.

Direct reprogramming of human somatic cells into pluripotency has broad implications in generating patient-specific induced pluripotent stem (iPS) cells for disease modeling and cellular replacement

Generation of human-induced pluripotent stem cells

This protocol describes how to establish primary human fibroblasts lines and how to derive iPS cells by retroviral transduction of reprogramming factors, and takes 2 months to complete reprograming human primary fibro Blasts starting from biopsy.

Generation of Human Induced Pluripotent Stem Cells from Umbilical Cord Matrix and Amniotic Membrane Mesenchymal Cells*

The efficient generation of induced pluripotent stem cells (iPSCs) from mesenchymal cells of the umbilical cord matrix and the placental amniotic membrane is reported using exogenous factors and a chemical mixture.

Feeder-free derivation of induced pluripotent stem cells from adult human adipose stem cells

It is shown here that induced pluripotent stem cells can be generated from adult human adipose stem cells (hASCs) freshly isolated from patients, and can be readily derived from adult hASCs in a feeder-free condition, thereby eliminating potential variability caused by using feeder cells.

Generation of induced pluripotent stem cells from a small amount of human peripheral blood using a combination of activated T cells and Sendai virus

A detailed protocol for the generation of iPSCs from a small amount of human peripheral blood using a combination of activated T cells and mutant SeV encoding human OCT3/4, SOX2, KLF4 and c-MYC is presented; T cell–derived iPSCS can be generated within 1 month of blood sampling.

Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes

It is shown that reprograming of juvenile human primary keratinocytes by retroviral transduction with OCT4, SOX2, KLF4 and c-MYC is at least 100-fold more efficient and twofold faster compared with reprogramming of human fibroblasts.

Mouse Meningiocytes Express Sox2 and Yield High Efficiency of Chimeras after Nuclear Reprogramming with Exogenous Factors*

It is demonstrated that cells from the mouse meningeal membranes express elevated levels of the embryonic master regulator Sox2 and are highly amenable to iPS, highlighting a population of somatic cells that are ready to undergo i PS, thus highlighting a very attractive cell type for iPS research and application.
...