Generation of human epidermal constructs on a collagen layer alone.

  title={Generation of human epidermal constructs on a collagen layer alone.},
  author={Federica Riva and Andrea Casasco and Emanuele Nespoli and Antonia Icaro Cornaglia and Marco Casasco and Angela Faga and Silvia Scevola and Giuliano Mazzini and Alberto Calligaro},
  journal={Tissue engineering},
  volume={13 11},
Because engineered tissues are designed for clinical applications in humans, a major problem is the contamination of cocultures and tissues by allogenic molecules used to grow stem cells in vitro. The protocols that are commonly applied to generate epidermal equivalents in vitro require the use of irradiated murine fibroblasts as a feeder layer for keratinocytes. In this study, we report a simple procedure for growing human keratinocytes, isolated from adult skin, to generate an epidermal… CONTINUE READING
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Human adipose tissue derived cells delay re- epithelialisation compared to skin fibroblasts in organotypic skin culture

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