Generation of histocompatible tissues using nuclear transplantation

@article{Lanza2002GenerationOH,
  title={Generation of histocompatible tissues using nuclear transplantation},
  author={Robert Lanza and Ho Yun Chung and James J. Yoo and Peter J. Wettstein and Catherine E Blackwell and Nancy D. Borson and er Terekhov Menaka C Thounaojam William H Hofmeister and Gunter Schuch and Shay Soker and Carlos Torres Moraes and Michael D West and Anthony Atala},
  journal={Nature Biotechnology},
  year={2002},
  volume={20},
  pages={689-696}
}
Nuclear transplantation (therapeutic cloning) could theoretically provide a limitless source of cells for regenerative therapy. Although the cloned cells would carry the nuclear genome of the patient, the presence of mitochondria inherited from the recipient oocyte raises questions about the histocompatibility of the resulting cells. In this study, we created bioengineered tissues from cardiac, skeletal muscle, and renal cells cloned from adult bovine fibroblasts. Long-term viability was… 

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References

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The review of nuclear transfer techniques to a range of mammalian species discusses recent progress that has been made as well as the inherent dangers and scientific challenges that remain before these techniques can be used to harness genetically matched cells and tissues for human transplantation.

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Although these ten sheep are authentic nuclear clones, they are in fact genetic chimaeras, containing somatic cell-derived nuclear DNA but oocyte-derived mtDNA, with the origin of the mtDNA of each of the ten nuclear-transfer sheep derived exclusively from recipient enucleated oocytes.

Transmitochondrial differences and varying levels of heteroplasmy in nuclear transfer cloned cattle

The results suggest that mtDNA type of donor embryos and recipient oocytes used in nuclear transfer cattle cloning should be controlled to obtain true clones with identical nuclear and cytoplasmic genomes.

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Applications of tissue-engineering techniques and gene therapy might allow the transfection of diseased tissues with designated cDNA to eliminate inherent or acquired defects in renal function replacement.

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Tests on proximal tubular epithelial cells (PTEC) and T cells bearing the interleukin 2 (IL-2) receptor from biopsies after transplantation showed that tubular damage by graft infiltrating cells (GIC) is a sign of clinically significant rejection.

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