Generation of histocompatible tissues using nuclear transplantation

  title={Generation of histocompatible tissues using nuclear transplantation},
  author={Robert Lanza and Ho Yun Chung and James J. Yoo and Peter J. Wettstein and Catherine E Blackwell and Nancy D. Borson and er Terekhov Menaka C Thounaojam William H Hofmeister and Gunter Schuch and Shay Soker and Carlos Torres Moraes and Michael D West and Anthony Atala},
  journal={Nature Biotechnology},
Nuclear transplantation (therapeutic cloning) could theoretically provide a limitless source of cells for regenerative therapy. Although the cloned cells would carry the nuclear genome of the patient, the presence of mitochondria inherited from the recipient oocyte raises questions about the histocompatibility of the resulting cells. In this study, we created bioengineered tissues from cardiac, skeletal muscle, and renal cells cloned from adult bovine fibroblasts. Long-term viability was… 

Production of cloned pigs from salivary gland-derived progenitor cells.

It is suggested that pSGPs represent appropriate donor cells for porcine somatic cell nuclear transfer and should be used for tissue stem cell transplantation in clinical settings.

Regeneration of the Infarcted Heart With Stem Cells Derived by Nuclear Transplantation

C-kit–positive stem cells derived by nuclear transfer cloning restore infarcted myocardium and the magnitude of myocardial regeneration obtained in this study is significantly superior to that achieved with adult bone marrow cells.

Tissue engineering, stem cells, cloning, and parthenogenesis: new paradigms for therapy

  • J. HippA. Atala
  • Biology, Medicine
    Journal of experimental & clinical assisted reproduction
  • 2004
Recent progress in tissue engineering is reviewed and applications of these new technologies that may offer novel therapies for patients with end-stage organ failure are described.

Survival of Skin Graft between Transgenic Cloned Dogs and Non-Transgenic Cloned Dogs

Results show that transplanting genetically- modified tissues into normal, syngeneic or genetically-modified recipient dogs with different mtDNA haplotypes do not elicit skin graft rejection or affect expression of the inserted gene, therefore, therapeutically valuable tissue derived from SCNT with genetic modification might be used safely in clinical applications for patients with diseased tissues.

Producing primate embryonic stem cells by somatic cell nuclear transfer

A modified SCNT approach is used to produce rhesus macaque blastocysts from adult skin fibroblasts, and two ES cell lines are isolated from these embryos, representing successful nuclear reprogramming of adult somatic cells into pluripotent ES cells and demonstrating proof-of-concept for therapeutic cloning in primates.

Tissue engineering, stem cells and cloning: current concepts and changing trends

  • A. Atala
  • Medicine, Biology
    Expert opinion on biological therapy
  • 2005
Recent advances in stem cell research and regenerative medicine are reviewed, and the clinical applications of these technologies as novel therapies for tissue or organ loss are described.

Organogenesis forum lecture: In vitro kidney development, tissue engineering and systems biology.

Strong evidence is provided that implantation of kidney-like tissue bioengineered from the recombination of in vitro culture systems which model discrete aspects of kidney development is possible, particularly in the setting of this novel and seemingly powerful xeno-based tissue engineering strategy.

Renal differentiation of amniotic fluid stem cells

It is shown that these pluripotent cells can develop and differentiate into de novo kidney structures during organogenesis in vitro, and may represent a potentially limitless source of ethically neutral, unmodified pluripotential cells for kidney regeneration.



Prospects for the use of nuclear transfer in human transplantation

The review of nuclear transfer techniques to a range of mammalian species discusses recent progress that has been made as well as the inherent dangers and scientific challenges that remain before these techniques can be used to harness genetically matched cells and tissues for human transplantation.

Mitochondrial DNA genotypes in nuclear transfer-derived cloned sheep

Although these ten sheep are authentic nuclear clones, they are in fact genetic chimaeras, containing somatic cell-derived nuclear DNA but oocyte-derived mtDNA, with the origin of the mtDNA of each of the ten nuclear-transfer sheep derived exclusively from recipient enucleated oocytes.

Transmitochondrial differences and varying levels of heteroplasmy in nuclear transfer cloned cattle

The results suggest that mtDNA type of donor embryos and recipient oocytes used in nuclear transfer cattle cloning should be controlled to obtain true clones with identical nuclear and cytoplasmic genomes.

Renal therapy using tissue-engineered constructs and gene delivery

Applications of tissue-engineering techniques and gene therapy might allow the transfection of diseased tissues with designated cDNA to eliminate inherent or acquired defects in renal function replacement.

Analysis of T cell lines from rejecting renal allografts.

Tests on proximal tubular epithelial cells (PTEC) and T cells bearing the interleukin 2 (IL-2) receptor from biopsies after transplantation showed that tubular damage by graft infiltrating cells (GIC) is a sign of clinically significant rejection.

Generation of T cells with lytic specificity for atypical antigens. I. A mitochondrial antigen in the rat

An antigen system most likely of mitochondrial origin with the following properties: it is transmitted maternally from DA strain females, inherited in an extra- chromosomal manner, restricted by class I RT1Aa major histocompatibility complex gene products, extinguished on target cells treated with chloramphenicol, and its pattern of expression in different rat strains correlates with restriction fragment-length polymorphisms of mitochondrial DNA.

Tubulogenesis from isolated single cells of adult mammalian kidney: clonal analysis with a recombinant retrovirus.

Individual cells in the adult kidney have retained the ability for kidney tubuleogenesis in vitro, since cell lineage analysis with a recombinant retrovirus demonstrated that in vitro tubulogenesis arose from clonal expansion of a single cell.

Somatic Cell Nuclear Transfer in Humans: Pronuclear and Early Embryonic Development

The ability to create autologous embryos represents the first step towards generating immune-compatible stem cells that could be used to overcome the problem of immune rejection in regenerative medicine.

Cloning of an endangered species (Bos gaurus) using interspecies nuclear transfer.

It is shown that interspecies nuclear transfer can be used to clone an endangered species with normal karyotypic and phenotypic development through implantation and the late stages of fetal growth, and suggests that somatic cell cloning methods could be use to restore endangered, or even extinct, species and populations.

The Ethical Reasons for Stem Cell Research

It is believed that urgent health needs provide strong moral grounds for pursuing ES cell research and that the United States is a religiously and ethically pluralistic nation, and at least three ethical considerations recommend federal funding for this research.