Generation of high-quality protein extracts from formalin-fixed, paraffin-embedded tissues.

@article{Addis2009GenerationOH,
  title={Generation of high-quality protein extracts from formalin-fixed, paraffin-embedded tissues.},
  author={Maria Filippa Addis and Alessandro Tanca and Daniela Pagnozzi and Salvatore Crobu and Giuseppe Fanciulli and Paolo Cossu-Rocca and Sergio Uzzau},
  journal={Proteomics},
  year={2009},
  volume={9 15},
  pages={3815-23}
}
A wealth of information on proteins involved in many aspects of disease is encased within formalin-fixed paraffin-embedded (FFPE) tissue repositories stored in hospitals worldwide. Recently, access to this "hidden treasure" is being actively pursued by the application of two main extraction strategies: digestion of the entangled protein matrix with generation of tryptic peptides, or decrosslinking and extraction of full-length proteins. Here, we describe an optimised method for extraction of… CONTINUE READING

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