Generation of high-affinity DNA aptamers using an expanded genetic alphabet

  title={Generation of high-affinity DNA aptamers using an expanded genetic alphabet},
  author={M. Kimoto and Rie Yamashige and Ken-ichiro Matsunaga and S. Yokoyama and I. Hirao},
  journal={Nature Biotechnology},
  • M. Kimoto, Rie Yamashige, +2 authors I. Hirao
  • Published 2013
  • Medicine, Biology
  • Nature Biotechnology
  • DNA aptamers produced with natural or modified natural nucleotides often lack the desired binding affinity and specificity to target proteins. Here we describe a method for selecting DNA aptamers containing the four natural nucleotides and an unnatural nucleotide with the hydrophobic base 7-(2-thienyl)imidazo[4,5-b]pyridine (Ds). We incorporated up to three Ds nucleotides in a random sequence library, which is expected to increase the chemical and structural diversity of the DNA molecules… CONTINUE READING
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