Generation of families of construct variants using golden gate shuffling.

@article{Engler2011GenerationOF,
  title={Generation of families of construct variants using golden gate shuffling.},
  author={Carola Engler and Sylvestre Marillonnet},
  journal={Methods in molecular biology},
  year={2011},
  volume={729},
  pages={167-81}
}
Current standard cloning methods based on the use of restriction enzymes and ligase are very versatile, but are not well suited for high-throughput cloning projects or for assembly of many DNA fragments from several parental plasmids in a single step. We have previously reported the development of an efficient cloning method based on the use of type IIs restriction enzymes and restriction-ligation. Such method allows seamless assembly of multiple fragments from several parental plasmids with… CONTINUE READING
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