Generation of double-stranded breaks in hypernegatively supercoiled DNA by Drosophila topoisomerase IIIbeta, a type IA enzyme.

Abstract

Drosophila topoisomerase (topo) IIIbeta is a member of the type IA family of DNA topoisomerases, which generates a single-stranded break to form a covalent complex with the 5'-end of DNA. We show here that a purified preparation of topo IIIbeta is able to convert a hypernegatively supercoiled substrate into primarily nicked, but also linear, DNA at enzyme… (More)

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Cite this paper

@article{WilsonSali2002GenerationOD, title={Generation of double-stranded breaks in hypernegatively supercoiled DNA by Drosophila topoisomerase IIIbeta, a type IA enzyme.}, author={Tina Wilson-Sali and Tao-shih Hsieh}, journal={The Journal of biological chemistry}, year={2002}, volume={277 30}, pages={26865-71} }