Generation of dendritic cell-like antigen-presenting cells in long-term mixed leucocyte culture: phenotypic and functional studies.

@article{Gao1997GenerationOD,
  title={Generation of dendritic cell-like antigen-presenting cells in long-term mixed leucocyte culture: phenotypic and functional studies.},
  author={Jingzhe Gao and Joaqu{\'i}n Madrenas and Wei-ping Zeng and Robert London Zhong and David Robert Grant},
  journal={Immunology},
  year={1997},
  volume={91 1},
  pages={
          135-44
        }
}
The mechanisms contributing to the proliferation and differentiation of antigen-presenting cell (APC) precursors upon antigen stimulation or tissue injury are poorly understood. Herein, we report the induction of a population of dendritic cell-like cells (DLC) with potent antigen-presentation function from unfractionated spleen cells by means of repetitive allostimulation in long-term mixed leucocyte cultures (LT-MLC). Initially, only a few adherent DLC were observed. By 4-6 weeks, however… CONTINUE READING
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