Generation of deletion and point mutations with one primer in a single cloning step.


Site-directed mutagenesis is an important technique for studying DNA and protein function. With the advent of PCR, several new approaches have been successfully developed (1,3,5–9). Early methods involved two-step PCR followed by subcloning, which is time consuming and error prone. Recently introduced site-directed mutagenesis kits such as QuikChangeTM Site… (More)


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