• Biology, Medicine
  • Published in
    The Plant journal : for cell…
    2017
  • DOI:10.1111/tpj.13319

Generation of chromosomal deletions in dicotyledonous plants employing a user‐friendly genome editing toolkit

@article{Ordon2017GenerationOC,
  title={Generation of chromosomal deletions in dicotyledonous plants employing a user‐friendly genome editing toolkit},
  author={Jana Ordon and Johannes Gantner and Jan Kemna and Lennart Schwalgun and Maik Reschke and Jana Streubel and Jens Boch and Johannes Stuttmann},
  journal={The Plant Journal},
  year={2017},
  volume={89},
  pages={155–168}
}
Genome editing facilitated by Cas9-based RNA-guided nucleases (RGNs) is becoming an increasingly important and popular technique for reverse genetics in both model and non-model species. So far, RGNs were mainly applied for the induction of point mutations, and one major challenge consists in the detection of genome-edited individuals from a mutagenized population. Also, point mutations are not appropriate for functional dissection of non-coding DNA. Here, the multiplexing capacity of a newly… CONTINUE READING

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