Generation of cell-free extracts of Xenopus eggs and demembranated sperm chromatin for the assembly and isolation of in vitro–formed nuclei for Western blotting and scanning electron microscopy (SEM)

@article{Allen2007GenerationOC,
  title={Generation of cell-free extracts of Xenopus eggs and demembranated sperm chromatin for the assembly and isolation of in vitro–formed nuclei for Western blotting and scanning electron microscopy (SEM)},
  author={Terry D. Allen and Sandra A. Rutherford and Steve Murray and Helen S. Sanderson and Fiona C Gardiner and Elena Kiseleva and Martin W. Goldberg and Sheona P. Drummond},
  journal={Nature Protocols},
  year={2007},
  volume={2},
  pages={1173-1179}
}
This protocol details methods for the generation of cell-free extracts and DNA templates from the eggs and sperm chromatin, respectively, of the clawed toad Xenopus laevis. We have used this system with scanning electron microscopy (SEM), as detailed herein, to analyze the biochemical requirements and structural pathways for the biogenesis of eukaryotic… CONTINUE READING