Generation of assays and antibodies to facilitate the study of human 5'-tyrosyl DNA phosphodiesterase.


Topoisomerases regulate DNA topology by the transient cleavage and religation of DNA during transcription and replication. Topoisomerase II (Topo II) poisons such as etoposide can induce abortive DNA strand breaks in which Topo II remains covalently bound to a 5' DNA strand terminus via a phosphotyrosyl linker. Tyrosyl DNA phosphodiesterase 2 (Tdp2) is a… (More)
DOI: 10.1016/j.ab.2013.02.001