Generation of an isogenic collection of yeast actin mutants and identification of three interrelated phenotypes.

@article{Whitacre2001GenerationOA,
  title={Generation of an isogenic collection of yeast actin mutants and identification of three interrelated phenotypes.},
  author={Johanna L Whitacre and Dana Davis and Kurt A. Toenjes and S M Brower and Andrea E. Adams},
  journal={Genetics},
  year={2001},
  volume={157 2},
  pages={533-43}
}
A large collection of yeast actin mutations has been previously isolated and used in numerous studies of actin cytoskeletal function. However, the various mutations have been in congenic, rather than isogenic, backgrounds, making it difficult to compare the subtle phenotypes that are characteristic of these mutants. We have therefore placed 27 mutations in an isogenic background. We used a subset of these mutants to compare the degree to which different actin alleles are defective in… CONTINUE READING

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