Generation of active oxygens, cell deformation and membrane potential changes upon UV-B irradiation in human blood cells.

@article{Ichiki1994GenerationOA,
  title={Generation of active oxygens, cell deformation and membrane potential changes upon UV-B irradiation in human blood cells.},
  author={H. Ichiki and Hirohumi Sakurada and Naoki Kamo and Tsuneo A. Takahashi and Sadayoshi Sekiguchi},
  journal={Biological & pharmaceutical bulletin},
  year={1994},
  volume={17 8},
  pages={1065-9}
}
The generation of peroxides (presumably hydrogen peroxide) by UV-B irradiation of human blood cells was detected. Non-fluorescent dihydrorhodamine 123 (DHR) is oxidized to fluorescent rhodamine 123 (R123) by H2O2 or peroxides with a stoichiometry of 1:1 in the presence of exogeneous peroxidase, and the fluorescence of R123 within the cells was measured using flow-cytometry. UV irradiation gave rise to changes in the cellular volume and the membrane potential, whose extent and direction were… CONTINUE READING

From This Paper

Topics from this paper.

Similar Papers

Loading similar papers…