Generation of a transgenic silkworm that secretes recombinant proteins in the sericin layer of cocoon: production of recombinant human serum albumin.

@article{Ogawa2007GenerationOA,
  title={Generation of a transgenic silkworm that secretes recombinant proteins in the sericin layer of cocoon: production of recombinant human serum albumin.},
  author={Shingo Ogawa and Masahiro Tomita and Katsuhiko Shimizu and Katsutoshi Yoshizato},
  journal={Journal of biotechnology},
  year={2007},
  volume={128 3},
  pages={531-44}
}
In this study we produced germline transgenic silkworms that spin cocoons containing recombinant human serum albumin (rHSA) in the sericin layer. A piggyBac-based transformation vector was constructed that carried HSA cDNA driven by sericin-1 gene promoter, viral enhancer hr3, and gene encoding viral trans-activator IE1. Isolated silk glands were bombarded with the vector and transplanted into host larvae. Three days later, the transplants were immunohistochemically analyzed, which showed that… CONTINUE READING
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