Generation of a stable folding intermediate which can be rescued by the chaperonins GroEL and GroES.

Abstract

Pig heart mitochondrial malate dehydrogenase was chemically denatured in guanidine HCl. Upon 50-fold dilution of the denaturant spontaneous refolding could be observed in the temperature range 12-32 degrees C. At 36 degrees C spontaneous refolding was not observed but a stable folding intermediate that is fairly resistant to aggregation was formed. This… (More)

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@article{Peralta1994GenerationOA, title={Generation of a stable folding intermediate which can be rescued by the chaperonins GroEL and GroES.}, author={Donna V Peralta and D. Hartman and Nick J Hoogenraad and Peter B. H\oj}, journal={FEBS letters}, year={1994}, volume={339 1-2}, pages={45-9} }