Generation of a soybean BAC library, and identification of DNA sequences tightly linked to the Rps1-k disease resistance gene

@article{Salimath1999GenerationOA,
  title={Generation of a soybean BAC library, and identification of DNA sequences tightly linked to the Rps1-k disease resistance gene},
  author={Shanmukhaswami S. Salimath and Madan K Bhattacharyya},
  journal={Theoretical and Applied Genetics},
  year={1999},
  volume={98},
  pages={712-720}
}
 A soybean bacterial artificial chromosome (BAC) library, comprising approximately 45 000 clones, was constructed from high-molecular-weight nuclear DNA of cultivar Williams 82, which carries the Rps1-k gene for resistance against Phytophthora sojae. The library is stored in 130 pools with about 350 clones per pool. Completeness of the library was evaluated for 21 random sequences including four markers linked to the Rps1 locus and 16 cDNAs. We identified pools containing BACs for all sequences… CONTINUE READING

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