Generation of a set of conditional analog-sensitive alleles of essential protein kinases in the fission yeast Schizosaccharomyces pombe.

@article{Cipak2011GenerationOA,
  title={Generation of a set of conditional analog-sensitive alleles of essential protein kinases in the fission yeast Schizosaccharomyces pombe.},
  author={Lubos Cipak and Chao Zhang and Ines Kov{\'a}cikov{\'a} and C. Raymond Rumpf and Eva Miadokov{\'a} and Kevan M. Shokat and Juraj Gregan},
  journal={Cell cycle},
  year={2011},
  volume={10 20},
  pages={
          3527-32
        }
}
The genome of the fission yeast Schizosaccharomyces pombe encodes for 17 protein kinases that are essential for viability. Studies of the essential kinases often require the use of mutant strains carrying conditional alleles. To inactivate these kinases conditionally, we applied a recently developed chemical genetic strategy. The mutation of a single residue in the ATP-binding pocket confers sensitivity to small-molecule inhibitors, allowing for specific inactivation of the modified kinase… CONTINUE READING
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