Generation of a selective 5-HT3B subunit-recognising polyclonal antibody; identification of immunoreactive cells in rat hippocampus

  title={Generation of a selective 5-HT3B subunit-recognising polyclonal antibody; identification of immunoreactive cells in rat hippocampus},
  author={Sarah A Monk and K N Desai and Catherine A. Brady and J. M. Williams and L. Princivalle and A Lin and Anthony G. Hope and Nicholas M Barnes},
Detection of human and rodent 5-HT3B receptor subunits by anti-peptide polyclonal antibodies
The generation and characterisation of a rabbit polyclonal antiserum that specifically recognises 5-HT3B receptor subunits is described and pAb77 antibodies are demonstrated to be useful for the determination of both receptor trafficking and also mapping 5- HT3 B receptor subunit expression in the CNS.
Immunolabelling of the 5‐HT3B receptor subunit in the central and peripheral nervous systems in rodents
Polyclonal antibodies directed against a portion of the second intracytoplasmic loop of the mouse 5‐HT3B subunit are developed and revealed that immunolabelling was most prominent in peripheral ganglia, particularly in trigeminal ganglia (TG).
5-HT3 receptors in the CNS: 3B or not 3B?
5-HT3 Receptors*
  • S. Lummis
  • Biology, Chemistry
    The Journal of Biological Chemistry
  • 2012
5-Hydroxytryptamine type 3 (5-HT3) receptors are cation-selective Cys loop receptors found in both the central and peripheral nervous systems and have potential for the treatment of a range of other conditions.
Molecular biology of 5-HT receptors
Subunit-Dependent Modulation of the 5-Hydroxytryptamine Type 3 Receptor Open-Close Equilibrium by n-Alcohols
The presence of the 5- HT33B subunit in 5-HT3AB receptors significantly reduces the enhancement of gating efficacy by small alcohols without altering the inhibitory actions of large alcohols.
5-HT3 receptors.
The 5-HT(3) receptor is a member of the Cys-loop family of ligand-gated ion channels that is located in both the peripheral and central nervous systems and the pharmacophore is reasonably well understood.
Quantitation of 5HT3 receptors in forebrain of serotonin transporter deficient mice
Using quantitative autoradiography, it is found that 5HT3 receptors are upregulated in frontal cortex, parietal cortex, and in stratum oriens of the CA3 region of the hippocampus of 5HTT knockout mice.
Serotonin 5-HT3 receptors in the central nervous system
It is speculated that 5-HT3 receptors play a role in the formation and function of cortical circuits as a neurotransmitter shaping development in the central nervous system.


The 5-HT3 Receptor Is Present in Different Subpopulations of GABAergic Neurons in the Rat Telencephalon
It is suggested that serotonin through the 5-HT3R may regulate GABA and CCK neurotransmission in the telencephalon and be selective within the GABA neuron population in the rat telENCEphalon.
The Pharmacological and Functional Characteristics of the Serotonin 5-HT3A Receptor Are Specifically Modified by a 5-HT3B Receptor Subunit*
The cloning of a subunit 5-HT3B with ∼44% amino acid identity to 5-ht3A that specifically modified 5- HT3Areceptor kinetics, voltage dependence, and pharmacology is reported, suggesting that 5-TT3B might contribute to tissue-specific functional changes in 5-Hat3-mediated signaling and/or modulation.
The 5-HT3B subunit is a major determinant of serotonin-receptor function
Surprisingly, the M2 region of the 5-HT3B subunit lacks any of the structural features that are known to promote the conductance of related receptors, and will be a valuable resource for defining the molecular mechanisms of ion-channel function.
Evidence for Expression of Heteromeric Serotonin 5‐HT3 Receptors in Rodents
Functional data and expression patterns are consistent with the existence of heteromeric 5‐HT3 receptors in rodent neurons, and sequence analysis and functional studies suggest that the new rodent subunits are orthologues of the human 5-HT3B subunit.
Purification of 5‐hydroxytryptamine3 receptors from porcine brain
The purification of the 5‐hydroxytryptamine3 (5‐HT3) receptor from a native tissue source, pig cerebral cortex, is demonstrated and it is suggested that this may contain non‐5‐ HT3‐A receptor subunit(s).
Functional properties of a cloned 5‐hydroxytryptamine ionotropic receptor subunit: comparison with native mouse receptors.
It is concluded that the receptor expressed from the cloned cDNA is indistinguishable from the 5‐HT3 receptor of N1E‐115 cells, suggesting an identical structure for these two receptors.