Generation of a recombinant avian coronavirus infectious bronchitis virus using transient dominant selection.

@article{Britton2005GenerationOA,
  title={Generation of a recombinant avian coronavirus infectious bronchitis virus using transient dominant selection.},
  author={Paul Britton and Sharon Evans and Brian Dove and Marc Davies and R C B Casais and David Cavanagh},
  journal={Journal of virological methods},
  year={2005},
  volume={123 2},
  pages={203-11}
}
A reverse genetics system for the avian coronavirus infectious bronchitis virus (IBV) has been described in which a full-length cDNA, corresponding to the IBV (Beaudette-CK) genome, was inserted into the vaccinia virus genome following in vitro assembly of three contiguous cDNAs [Casais, R., Thiel, V., Siddell, S.G., Cavanagh, D., Britton, P., 2001. Reverse genetics system for the avian coronavirus infectious bronchitis virus. J. Virol. 75, 12359-12369]. The method has subsequently been used to… CONTINUE READING