Generation of a packaging cell line for prolonged large-scale production of high-titer HIV-1-based lentiviral vector.

@article{Ni2005GenerationOA,
  title={Generation of a packaging cell line for prolonged large-scale production of high-titer HIV-1-based lentiviral vector.},
  author={Yajin Ni and Susan Sun and Ibe Oparaocha and Laurent Humeau and Brian Davis and Reuben A. Cohen and Gwendolyn Binder and Yung-Nien Chang and Vladimir Slepushkin and Boro Dropuli{\'c}},
  journal={The journal of gene medicine},
  year={2005},
  volume={7 6},
  pages={818-34}
}
BACKGROUND A stable packaging cell line facilitates large-scale lentivirus vector manufacture. However, it has been difficult to produce clinical-scale HIV-1-based lentiviral vectors using a packaging cell line, in part due to toxicity of packaging genes, and gene silencing that occurs during the long culture period necessary for sequential addition of packaging constructs. METHODS To avoid these problems, we developed a three-level cascade gene regulation system designed to remove… CONTINUE READING