Generation of a non-leaky heat shock-inducible Cre line for conditional Cre/lox strategies in zebrafish.

@article{Hans2011GenerationOA,
  title={Generation of a non-leaky heat shock-inducible Cre line for conditional Cre/lox strategies in zebrafish.},
  author={Stefan Hans and Dorian Freudenreich and Michaela Geffarth and Jan Kaslin and Anja Machate and Michael Brand},
  journal={Developmental dynamics : an official publication of the American Association of Anatomists},
  year={2011},
  volume={240 1},
  pages={
          108-15
        }
}
  • Stefan Hans, Dorian Freudenreich, +3 authors Michael Brand
  • Published in
    Developmental dynamics : an…
    2011
  • Medicine, Biology
  • Cre-mediated site-specific recombination has emerged as an indispensable tool for the precise manipulation of the mammalian genome. Recently, we showed that Cre is also highly efficient in zebrafish and temporal control of recombination can be achieved by using the ligand-inducible CreER(T2). Previous attempts have been made to control recombination by using the temperature inducible hsp70l promoter to conditionally drive the expression of Cre or EGFP-Cre, respectively. However, in this study… CONTINUE READING

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