Generation of a human melanocyte cell line by introduction of HPV16 E6 and E7 genes

  title={Generation of a human melanocyte cell line by introduction of HPV16 E6 and E7 genes},
  author={I. Poole and F. M. Berg and R. V. D. Wijngaard and D. Galloway and P. Amstel and A. Buffing and H. L. Smits and W. Westerhof and P. Das},
  journal={In Vitro Cellular & Developmental Biology - Animal},
  • I. Poole, F. M. Berg, +6 authors P. Das
  • Published 1997
  • Biology, Medicine
  • In Vitro Cellular & Developmental Biology - Animal
  • SummaryAvailability of a standard human melanocyte cell line with unlimited growth potential and otherwise normal melanocytic properties will greatly facilitate research in melanocyte biology and in vitro studies on the etiology of pigmentary disorders and melanoma. Using a retroviral vector, E6 and E7 open reading frames of human papilloma virus type 16 (HPV 16) have been introduced into cultured normal human melanocytes. Cells selected by increased resistance to geneticin conveyed by the… CONTINUE READING

    Figures, Tables, and Topics from this paper.

    Transformation and Immortalization
    • 4


    Publications referenced by this paper.
    Immortalization of primary human smooth muscle cells.
    • 109
    • PDF
    Selective proliferation of normal human melanocytes in vitro in the presence of phorbol ester and cholera toxin.
    • 509
    • Highly Influential
    • PDF