Generation of Rye (Secale cereale L.) Plants With Low Transgene Copy Number After Biolistic Gene Transfer and Production of Instantly Marker-Free Transgenic Rye

@article{Popelka2003GenerationOR,
  title={Generation of Rye (Secale cereale L.) Plants With Low Transgene Copy Number After Biolistic Gene Transfer and Production of Instantly Marker-Free Transgenic Rye},
  author={Juan Carlos Popelka and Jianping Xu and Fredy Altpeter},
  journal={Transgenic Research},
  year={2003},
  volume={12},
  pages={587-596}
}
Rye is extremely recalcitrant to tissue culture and genetic transformation. We describe the efficient and reproducible production of stably expressing transgenic rye plants after biolistic gene transfer to callus tissue derived from immature embryos. Key factors in the production of transgenic rye plants include the identification of biolistic gene transfer parameters and a selection protocol, which does not affect its regeneration ability. The bar gene was used as a selectable marker and… CONTINUE READING

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