Generation of Mouse and Human Induced Pluripotent Stem Cells (iPSC) from Primary Somatic Cells

  title={Generation of Mouse and Human Induced Pluripotent Stem Cells (iPSC) from Primary Somatic Cells},
  author={I. Lorenzo and Aarne Fleischer and Daniel Bachiller},
  journal={Stem Cell Reviews and Reports},
Cellular reprogramming consists of the conversion of differentiated cells into pluripotent cells; the so-called induced Pluripotent Stem Cells. iPSC are amenable to in vitro manipulation and, in theory, direct production of any differentiated cell type. Furthermore, iPSC can be obtained from sick individuals and subsequently used for disease modeling, drug discovery and regenerative treatments. iPSC production was first achieved by transducing, with the use of retroviral vectors, four specific… 

Generation of Induced Pluripotent Stem Cells from Human Epidermal Keratinocytes.

The research provides an easy and efficient method for producing iPSCs from keratinocytes, which has important applications in cell replacement therapy and demonstrates reduced DNA methylation compared with the original cells.

Continuous passages accelerate the reprogramming of mouse induced pluripotent stem cells.

The research shows that continuous passage increases the efficiency of reprogramming and generates more nearly fully reprogrammed iPSCs efficiently and rapidly, suggesting that full reprograming is a gradual process that does not merely end at the point of the activation of endogenous pluripotency-associated genes.

Production of Transgenic Porcine Embryos Reconstructed with Induced Pluripotent Stem-Like Cells Derived from Porcine Endogenous Factors Using piggyBac System.

The iPS-like cell line, generated from porcine transcriptional factors using the PB transposon system, demonstrated pluripotency with the capacity for unlimited self-renewal, and could be used as donor cells to produce cloned embryos by SCNT.

Generation of disease-specific induced pluripotent stem cells from patients with rheumatoid arthritis and osteoarthritis

FLSs derived from RA and OA could be cell resources for iPSC reprogramming and shown to be compatible by karyotyping, and have the potential to be applied in clinical settings as source materials for molecular diagnosis and regenerative therapy.

AB0144 Generation of Disease-Specific Induced Pluripotent Stem Cells from Patients with Rheumatoid Arthritis and Osteoarthritis

FLSs derived from RA and OA could be cell resources for iPSC reprogramming and shown to be compatible by karyotyping, and disease- and patient-specific iPSCs have the potential to be applied in clinical settings as source materials for molecular diagnosis and regenerative therapy.

Generation of Mouse Pluripotent Stem Cell–Derived Proliferating Myeloid Cells as an Unlimited Source of Functional Antigen-Presenting Cells

Mouse proliferating myeloid cells exhibiting cytokine-dependent proliferation and DC-like differentiation may be used to address issues associated with the preparation of DCs.

A Review: Induced Pluripotent Stem Cells (IPSC) and Their Reprogramming Techniques

It has been an incredible journey of stem cells from embryonic stem cells to induced pluripotent stem cells, and people consider stem cell therapy as an initial step for personalized medicine; they also see it like a safety deposit to the upcoming future.

Generation of induced pluripotent stem cells from neonatal mouse cochlear cells.

The Use of Trichostatin A during Pluripotent Stem Cell Generation Does Not Affect MHC Expression Level

It is concluded that the use of short-term and low concentration of TSA during reprogramming in PSC generation procedure significantly increases P SC generation efficiency, but do not affect the MHC expression in established cell lines, which is in the benefit of cell transplantation in regenerative medicine.

Utilising Induced Pluripotent Stem Cells in Neurodegenerative Disease Research: Focus on Glia

Using glial cells derived from human iPSCs in in vitro cell culture, cerebral organoids, and intracranial transplantation may give future insight into both more accurate models and disease-modifying therapies.



Generation of human-induced pluripotent stem cells

This protocol describes how to establish primary human fibroblasts lines and how to derive iPS cells by retroviral transduction of reprogramming factors, and takes 2 months to complete reprograming human primary fibro Blasts starting from biopsy.

Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts

A modified protocol for the generation of iPS cells that does not require the Myc retrovirus is described and, with this protocol, significantly fewer non-iPS background cells are obtained, and theiPS cells generated were consistently of high quality.

Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2

Valproic acid (VPA), a histone deacetylase inhibitor, enables reprogramming of primary human fibroblasts with only two factors, Oct4 and Sox2, without the need for the oncogenes c-Myc or Klf4, and supports the possibility of reprograming through purely chemical means.

Induced Pluripotent Stem Cell Generation Using a Single Lentiviral Stem Cell Cassette

Using a single lentiviral vector expressing a “stem cell cassette” composed of the four transcription factors and a combination of 2A peptide and internal ribosome entry site technology achieves the most efficient reprogramming system to date and allows derivation of iPS cells with a single viral integration.

Generation of Mouse Induced Pluripotent Stem Cells Without Viral Vectors

The production of virus-free iPS cells, albeit from embryonic fibroblasts, addresses a critical safety concern for potential use of i PS cells in regenerative medicine.

Human Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences

Results demonstrate that reprograming human somatic cells does not require genomic integration or the continued presence of exogenous reprogramming factors and removes one obstacle to the clinical application of human iPS cells.

Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes

It is shown that reprograming of juvenile human primary keratinocytes by retroviral transduction with OCT4, SOX2, KLF4 and c-MYC is at least 100-fold more efficient and twofold faster compared with reprogramming of human fibroblasts.

Virus free induction of pluripotency and subsequent excision of reprogramming factors

It is shown that non-viral transfection of a single multiprotein expression vector, which comprises the coding sequences of c-Myc, Klf4, Oct4 and Sox2 linked with 2A peptides, can reprogram both mouse and human fibroblasts and can be removed once reprogramming has been achieved.

iPS cells produce viable mice through tetraploid complementation

The generation of several iPS cell lines that are capable of generating viable, fertile live-born progeny by tetraploid complementation and confirm that iPS cells can attain true pluripotency that is similar to that of ES cells.