Generation of Mouse Induced Pluripotent Stem Cells Without Viral Vectors

@article{Okita2008GenerationOM,
  title={Generation of Mouse Induced Pluripotent Stem Cells Without Viral Vectors},
  author={K. Okita and M. Nakagawa and Hong Hyenjong and T. Ichisaka and S. Yamanaka},
  journal={Science},
  year={2008},
  volume={322},
  pages={949 - 953}
}
Induced pluripotent stem (iPS) cells have been generated from mouse and human somatic cells by introducing Oct3/4 and Sox2 with either Klf4 and c-Myc or Nanog and Lin28 using retroviruses or lentiviruses. Patient-specific iPS cells could be useful in drug discovery and regenerative medicine. However, viral integration into the host genome increases the risk of tumorigenicity. Here, we report the generation of mouse iPS cells without viral vectors. Repeated transfection of two expression… Expand

Paper Mentions

Interventional Clinical Trial
The investigators plan to obtain skin and blood samples from healthy volunteers and patients with a benign, inherited hematologic disease to use for research to use homologous… Expand
ConditionsAnemia, Sickle Cell
InterventionProcedure
Generation of mouse-induced pluripotent stem cells by transient expression of a single nonviral polycistronic vector
Generation of iPSCs from mouse fibroblasts with a single gene, Oct4, and small molecules
Emerging methods for preparing iPS cells.
Integration-free Methods for Generating Induced Pluripotent Stem Cells
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References

SHOWING 1-10 OF 20 REFERENCES
Generation of human induced pluripotent stem cells from dermal fibroblasts
...
1
2
...