Generation of Long Insert Pairs Using a Cre-LoxP Inverse PCR Approach

Abstract

Large insert mate pair reads have a major impact on the overall success of de novo assembly and the discovery of inherited and acquired structural variants. The positional information of mate pair reads generally improves genome assembly by resolving repeat elements and/or ordering contigs. Currently available methods for building such libraries have one or… (More)
DOI: 10.1371/journal.pone.0029437

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