Generation of Gene-Modified Cynomolgus Monkey via Cas9/RNA-Mediated Gene Targeting in One-Cell Embryos

@article{Niu2014GenerationOG,
  title={Generation of Gene-Modified Cynomolgus Monkey via Cas9/RNA-Mediated Gene Targeting in One-Cell Embryos},
  author={Yuyu Niu and Bin Shen and Yiqiang Cui and Yongchang Chen and Jianying Wang and Lei Wang and Yu Kang and Xiaoyang Zhao and Wei Si and Wei Li and Andy Peng Xiang and Jiankui Zhou and Xuejiang Guo and Ye Bi and Chenyang Si and Bian Hu and Guoying Dong and Hong Wang and Zuo-min Zhou and Tianqing Li and Tao Tan and Xiuqiong Pu and Fang Wang and Shaohui Ji and Qi Zhou and Xingxu Huang and Weizhi Ji and Jiahao Sha},
  journal={Cell},
  year={2014},
  volume={156},
  pages={836-843}
}
Monkeys serve as important model species for studying human diseases and developing therapeutic strategies, yet the application of monkeys in biomedical researches has been significantly hindered by the difficulties in producing animals genetically modified at the desired target sites. Here, we first applied the CRISPR/Cas9 system, a versatile tool for editing the genes of different organisms, to target monkey genomes. By coinjection of Cas9 mRNA and sgRNAs into one-cell-stage embryos, we… 
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