Generation of ES cells for conditional expression of nuclear receptors and coregulators in vivo.


Nuclear receptors and coregulators orchestrate diverse aspects of biological functions and inappropriate expression of these factors often associates with human diseases. The present study describes a conditional overexpression system consisting of a minigene located at the Rosa26 locus in the genome of mouse embryonic stem (ES) cells. Before activation… (More)
DOI: 10.1210/me.2010-0068