Generation of Dispersed Presomitic Mesoderm Cell Cultures for Imaging of the Zebrafish Segmentation Clock in Single Cells

@inproceedings{Webb2014GenerationOD,
  title={Generation of Dispersed Presomitic Mesoderm Cell Cultures for Imaging of the Zebrafish Segmentation Clock in Single Cells},
  author={Alexis B. Webb and Daniele Soroldoni and Annelie Oswald and Johannes E. Schindelin and Andrew C Oates},
  booktitle={Journal of visualized experiments : JoVE},
  year={2014}
}
Segmentation is a periodic and sequential morphogenetic process in vertebrates. This rhythmic formation of blocks of tissue called somites along the body axis is evidence of a genetic oscillator patterning the developing embryo. In zebrafish, the intracellular clock driving segmentation is comprised of members of the Her/Hes transcription factor family organized into negative feedback loops. We have recently generated transgenic fluorescent reporter lines for the cyclic gene her1 that… CONTINUE READING