Generation of CRISPR/Cas9-mediated gene-targeted pigs via somatic cell nuclear transfer

  title={Generation of CRISPR/Cas9-mediated gene-targeted pigs via somatic cell nuclear transfer},
  author={Xiaoqing Zhou and Jige Xin and Nana Fan and Qingjian Zou and Jiao Huang and Zhen Ouyang and Yu Zhao and Bentian Zhao and Zhaoming Liu and Sisi Lai and Xiaoling Yi and Lin Guo and Miguel A Esteban and Yangzhi Zeng and Huaqiang Yang and Liangxue Lai},
  journal={Cellular and Molecular Life Sciences},
The domestic pig has been widely used as an important large animal model. Precise and efficient genetic modification in pig provides a great promise in biomedical research. Recently, clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) system has been successfully used to produce many gene-targeted animals. However, these animals have been generated by co-injection of Cas9 mRNA and single-guide RNA (sgRNA) into one-cell stage embryos, which mostly resulted… CONTINUE READING
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CRISPR/Cas9-mediated gene-targeted pigs

  • FA Ran, PD Hsu, +8 authors F Zhang
  • 2013
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