Generation of C3a anaphylatoxin from human C3 by human mast cell tryptase.

@article{Schwartz1983GenerationOC,
  title={Generation of C3a anaphylatoxin from human C3 by human mast cell tryptase.},
  author={Lawrence B. Schwartz and Masaaki Kawahara and Tony E. Hugli and D P Vik and Douglas T Fearon and K Frank Austen},
  journal={Journal of immunology},
  year={1983},
  volume={130 4},
  pages={1891-5}
}
Tryptase, the dominant neutral protease of human pulmonary mast cell secretory granules, has the capacity in vitro to generate C3a anaphylatoxin from purified human C3. Only the alpha-chain of C3 is cleaved, and major fragments with apparent m.w. of 105,000, 39,500, 34,000, 29,000, and 9000 are detected by sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis under reducing conditions. Fragments of 34,000 and 9000 m.w. are detected without reduction. A portion of the 9000 m.w. protein… CONTINUE READING