Generation and synchronization of gonadotropin-releasing hormone (GnRH) pulses: intrinsic properties of the GT1-1 GnRH neuronal cell line.

@article{Escalera1992GenerationAS,
  title={Generation and synchronization of gonadotropin-releasing hormone (GnRH) pulses: intrinsic properties of the GT1-1 GnRH neuronal cell line.},
  author={Gonzalo Mart{\'i}nez de la Escalera and Anna L Choi and Richard I. Weiner},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1992},
  volume={89 5},
  pages={1852-5}
}
The immortalized neuronal cell line GT1-1 was used to investigate the endogenous pattern of GnRH release. The GT1-1 cell line was derived from a GnRH-secreting tumor in a transgenic mouse induced by genetically targeted expression of the potent simian virus 40 oncogene encoding tumor antigen. Cells attached to coverslips were superfused in Sykes-Moore chambers with Locke's medium, Ca(2+)-free Locke's medium, or Opti-MEM (another defined medium) for 2 hr, and samples were collected at 4-min… CONTINUE READING

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